Atong: Systematic genetic analysis with ordered arrays of yeast deletion
Tong et. al. (2004).
Description
Data from Tong et. al. (2004) buffering experiments using ordered
arrays of yeast deletion design by Tong et. al. (2001).
Usage
data(Atong) data(tong2004raw)
Format
tong2004Raw is dataframe extracted from Table S1 of
Tong et al. (2004) online supporting material. We added an extra column,
queryGene.sysName, which is the systematic names of the query genes.
- queryGene.geneName
- Column indicates the gene used as query in
the synthetic genetic array screen (SGA).
- Int.geneName
- Column indicates the gene identified as an
interactor with a particular query.
- Int.sysName
- Column indicates the systematic name of the open
reading frame (ORF) that corresponds to the interactor gene.
- Score
- An interaction scored three times in the three runs by
visual inspection received a scored of 3. An interaction scored twice
in the three in the runs by visual inspection received a scored of 2.
An interaction scored by the computer-based image analysis but not
visual inspection received a scored of 1. For interactions that scored
once in the three runs by visual inspection confirmation was attempted
only for those genes pairs related functions. Such confirmed
interactions received a score of 0.
- RSA
- Column identifies an interaction that was confirmed by random
spore analysis.
- Tetrad
- Column identifies an interaction confirmed by tetrad analysis.
- SS
- Refers to synthetic sick interaction.
- SL
- Refers to synthetic lethal interaction.
- Functional.Role
- Column indicates the assigned GO functional
annotation from their defined subset of annotations.All the
interactions are identified in this study unless otherwise stated.
- References
- Genetic Interactions that have been previously
described.
- queryGene.sysName
- Column indicates the systematic (ORF) name of
the gene used as query in a SGA screen.
Atong is a 132 by 1008 adjacency matrix of the systematic genetic
interactions identified between 132 query genes and the deletion gene
set (Tong et al. 2001; see SGA for more details).
The row names correspond to the systematic (ORF) names for the 132 query
genes. The column names correspond to the systematic (ORF) names of the
1011 reporter genes, which showed a synthetic lethal or synthetic sick
interaction with at least one query genes. Values are 0 or 1, with a 1
indicating the occurrence of the genetic interaction between the gene pairs.Source
Tong et al, Science. Vol.303, 2004.