relQuantPCR: Compute relative expression values for realtime quantitative RT-PCR data
Description
Compute relative expression values for realtime quantitative RT-PCR data based on
Ct or take-off values, respectively. The computations use the PCR efficiency.
Usage
relQuantPCR(x, E = 2, na.rm = FALSE)
Arguments
x
numeric vector containing raw data
E
PCR efficiency
na.rm
a logical value indicating whether NA values should be
stripped before the computation proceeds.
Value
vector of relative expression values w.r.t. specified PCR efficiency.
References
Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002). Accurate
normalization of real-time quantitative RT-PCR data by geometric averaging
of multiple internal control genes.
Genome Biology 2002. 3(7):research0034.1-0034.11.
http://genomebiology.com/2002/3/7/research/0034/