SWATH2stats (version 1.2.3)

assess_fdr_byrun: Assess assay, peptide and protein level FDR by run (for each MS_injection separately) in OpenSWATH output table

Description

This function estimates the assay, peptide and protein FDR by run in an OpenSWATH result table in dependence of a range of m_score cutoffs. The results can be visualized and summarized by the associated method plot.fdr_table(). It counts target and decoy assays (unique transition_group_id), peptides (unique FullPeptideName) and proteins (unique ProteinName) in the OpenSWATH output table in dependence of m-score cutoff, the useful m_score cutoff range is evaluated for each dataset individually on the fly.

To arrive from decoy counts at an estimation of the false discovery rate (false positives among the targets remaining at a given mscore cutoff) the ratio of false positives to true negatives (decoys) (FFT) must be supplied. It is estimated for each run individually by pyProphet and contained in the pyProphet statistics [Injection_name]_full_stat.csv. As an approximation, the FFTs of multiple runs are averaged and supplied as argument FFT. For further details see the Vignette Section 1.3 and 4.1.

To assess fdr over the entire dataset, please refer to function assess_fdr_overall.

FDR is calculated as FDR = (TN*FFT/T); TN=decoys, T=targets, FFT=see above

Usage

assess_fdr_byrun(data, FFT, n.range = 20, output = "pdf_csv", plot = TRUE, 
filename = "FDR_report_byrun")

Arguments

data

Annotated OpenSWATH/pyProphet output table. Refer to function sample_annotation from this package for further information.

FFT

Ratio of false positives to true negatives, q-values from [Injection_name]_full_stat.csv in pyProphet stats output. As an approximation, the q-values of multiple runs are averaged and supplied as argument FFT. Numeric from 0 to 1. Defaults to 1, the most conservative value (1 Decoy indicates 1 False target).

n.range

Option to set the number of magnitude for which the m_score threshold is decreased (e.g. n.range = 10, m-score from 0.1 until 10^-10)^.

output

Choose output type. "pdf_csv" creates the output as files in the working directory, "Rconsole" triggers delivery of the output to the console enabling further computation or custom plotting / output.

plot

Logical, whether or not to create plots from the results (using the associated method plot.fdr_cube()

filename

Optional, modifying the basename of the result files if applicable.

Value

Returns an array of target/decoy identification numbers and calculated FDR values at different m-score cutoffs.

Examples

data("OpenSWATH_data", package="SWATH2stats")
data("Study_design", package="SWATH2stats")
data <- sample_annotation(OpenSWATH_data, Study_design)
assess_fdr_byrun(data, FFT=0.7, output = "pdf_csv", plot = TRUE, 
filename="Testoutput_assess_fdr_byrun")