
Last chance! 50% off unlimited learning
Sale ends in
Score cell cycle phases
CellCycleScoring(
object,
s.features,
g2m.features,
ctrl = NULL,
set.ident = FALSE,
...
)
A Seurat object with the following columns added to object meta data: S.Score, G2M.Score, and Phase
A Seurat object
A vector of features associated with S phase
A vector of features associated with G2M phase
Number of control features selected from the same bin per
analyzed feature supplied to AddModuleScore
.
Defaults to value equivalent to minimum number of features
present in 's.features' and 'g2m.features'.
If true, sets identity to phase assignments Stashes old identities in 'old.ident'
Arguments to be passed to AddModuleScore
AddModuleScore
if (FALSE) {
data("pbmc_small")
# pbmc_small doesn't have any cell-cycle genes
# To run CellCycleScoring, please use a dataset with cell-cycle genes
# An example is available at http://satijalab.org/seurat/cell_cycle_vignette.html
pbmc_small <- CellCycleScoring(
object = pbmc_small,
g2m.features = cc.genes$g2m.genes,
s.features = cc.genes$s.genes
)
head(x = pbmc_small@meta.data)
}
Run the code above in your browser using DataLab