TmCalculator (version 1.0.0)

Tm_Wallace: Calculate the melting temperature using the 'Wallace rule'

Description

The Wallace rule is often used as rule of thumb for approximate melting temperature calculations for primers with 14 to 20 nt length.

Usage

Tm_Wallace(ntseq, ambiguous = FALSE)

Arguments

ntseq

The primer/probe sequence as string or vector of characters

ambiguous

Ambiguous bases are taken into account to compute the G and C content when ambiguous is TRUE.

Value

a numeric melting temperature

References

Thein S L , Lynch J R , Weatherall D J , et al. DIRECT DETECTION OF HAEMOGLOBIN E WITH SYNTHETIC OLIGONUCLEOTIDES[J]. The Lancet, 1986, 327(8472):93.

Examples

# NOT RUN {
ntseq = c('acgtTGCAATGCCGTAWSDBSY') #for wallace rule
Tm_Wallace(ntseq,ambiguous = TRUE)
Tm_Wallace(ntseq,ambiguous = FALSE)
# }