Sequence (5' to 3') of one strand of the DNA nucleic acid duplex
as string or vector of characters (Note: Non-DNA characters are ignored
by this method).
ambiguous
Ambiguous bases are taken into account to compute the G and C content when ambiguous is TRUE.
outlist
output a list of Tm and options or only Tm value, default is TRUE.
References
Thein S L , Lynch J R , Weatherall D J , et al. DIRECT DETECTION OF HAEMOGLOBIN E WITH SYNTHETIC OLIGONUCLEOTIDES[J]. The Lancet, 1986, 327(8472):93.