TransProR (version 1.0.2)

Wilcoxon_analyze: Differential Gene Expression Analysis Using Wilcoxon Rank-Sum Test

Description

This function performs differential gene expression analysis using Wilcoxon rank-sum tests. It reads tumor and normal expression data, performs TMM normalization using 'edgeR', and uses Wilcoxon rank-sum tests to identify differentially expressed genes.

Usage

Wilcoxon_analyze(
  tumor_file,
  normal_file,
  output_file,
  logFC_threshold = 2.5,
  fdr_threshold = 0.05
)

Value

A data frame of differential expression results.

Arguments

tumor_file

Path to the tumor data file (RDS format).

normal_file

Path to the normal data file (RDS format).

output_file

Path to save the output DEG data (RDS format).

logFC_threshold

Threshold for log fold change for marking up/down-regulated genes.

fdr_threshold

Threshold for FDR for filtering significant genes.

References

Li, Y., Ge, X., Peng, F., Li, W., & Li, J. J. (2022). Exaggerated False Positives by Popular Differential Expression Methods When Analyzing Human Population Samples. Genome Biology, 23(1), 79. DOI: https://doi.org/10.1186/s13059-022-02648-4.

Examples

# Define file paths for tumor and normal data from the data folder
tumor_file <- system.file("extdata",
                          "removebatch_SKCM_Skin_TCGA_exp_tumor_test.rds",
                          package = "TransProR")
normal_file <- system.file("extdata",
                           "removebatch_SKCM_Skin_Normal_TCGA_GTEX_count_test.rds",
                           package = "TransProR")
output_file <- file.path(tempdir(), "Wilcoxon_rank_sum_testoutRst.rds")

# Run the Wilcoxon rank sum test
outRst <- Wilcoxon_analyze(
  tumor_file = tumor_file,
  normal_file = normal_file,
  output_file = output_file,
  logFC_threshold = 2.5,
  fdr_threshold = 0.01
)

# View the top 5 rows of the result
head(outRst, 5)