mobData: Data from a set of small RNA sequencing experiments carried out
on grafts of Arabidopsis thaliana intended for differential expression
analyses.
Description
This data set is a matrix (`mobData') of counts acquired
for three thousand small RNA loci from a set of Arabidopsis grafting
experiments. Three different biological conditions exist within these
data; one in which a Dicer 2,3,4 triple mutant shoot is grafted onto a
Dicer 2,3,4 triple mutant root (SL236 and SL260), one
in which a wild-type shoot is grafted onto a wild-type root
(SL239 and SL240), and one in which a wild-type shoot
is grafted onto a Dicer 2,3,4 triple mutant root (SL237 and
SL238). Dicer 2,3,4 is required for the production of 22nt
and 24nt small RNAs, as well as some 21nt ones. Consequently, if we
detect differentially expressed sRNA loci in the root stock of the
grafts, we can make inferences about the mobility of small RNAs. The annotation of the loci from which these data derive is in data
file `mobAnnotation'.
Format
A matrix of which each of the six columns represents a sample,
and each row an sRNA locus (acquired by sequencing).Source
Illumina sequencing.References
Molnar A. and Melnyk C.W. et al. Small silencing RNAs in
plants are mobile and direct epigenetic modification in recipient
cells. Science (2010)