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beadarray (version 2.20.0)

Quality assessment and low-level analysis for Illumina BeadArray data

Description

The package is able to read bead-level data (raw TIFFs and text files) output by BeadScan as well as bead-summary data from BeadStudio. Methods for quality assessment and low-level analysis are provided.

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Version

Version

2.20.0

License

GPL-2

Maintainer

Mark Dunning

Last Published

February 15th, 2017

Functions in beadarray (2.20.0)

Annotation

Storage of annotation information
boxplot-methods

Boxplots from summary data
BASHExtended

BASH - Extended Defect Analysis
beadarrayUsersGuide

View beadarray User's Guide
backgroundCorrectSingleSection

Background correct an array-section
BASH

BASH - BeadArray Subversion of Harshlight
addFeatureData

Add probe data
BASHCompact

BASH - Compact Defect Analysis
BASHDiffuse

BASH - Diffuse Defect Analysis
beadarray-package

The beadarray package: a tool for low-level analysis of Illumina BeadArrays
beadRegistrationData-class

Class "beadRegistrationData"
limmaResults-class

Class "limmaResults"
ExpressionSetIllumina-class

Class "ExpressionSetIllumina"
checkRegistration

Perform check for misregistered array segments.
illuminaChannel-class

Class "illuminaChannel"
BeadLevelList-class

Class "BeadLevelList"
calculateDetection

Calculate detection scores
calculateOutlierStats

Outlier distribution stats
combine

Combine two objects.
controlProbeDetection

Percentage of beads detected
generateNeighbours

Generate matrix of neighbouring beads
makeGEOSubmissionFiles

Create files for a Gene Expression Omnibus submission
convertBeadLevelList

Convert a BeadLevelList object into a beadLevelData object
expressionQCPipeline

Flexible bead-level QC pipeline
metaTemplate

GEO required fields
dim

Retrieve the dimensions of an object
deprecatedFunctions

Deprecated Functions
HULK

HULK - Bead Array Normalization by NEighbourhood Residuals
getBeadData

Get raw data from a beadLevelData object
createTargetsFile

A function to generate a targets file given a directory of Illumina bead-level files
illuminaOutlierMethod

Identifier outliers on an array section
identifyControlBeads

Classify each bead according to its control status
insertSectionData

Modify the sectionData slot
limmaDE

Differential expression using limma
insertBeadData

Add, modify or remove data in a beadLevelData object
makeQCTable

Tabulate QC scores
plotMA-methods

Function to construct the classic MA plots from a dataset
imageplot

imageplot for beadLevelData object
imageProcessing

Image processing functions
makeQCProfile

Retrieve control beads
noOutlierMethod

returns no outliers on an array section
normaliseIllumina

Normalise Illumina expression data
outlierplot

Plot outlier locations
medianNormalise

Median normalise data in a matrix
plotChipLayout

Function to Plot the Layout of an Illumina BeadChip
platformSigs

Annotation definitions
metrics-methods

Accessing metrics information in bead-level objects
beadIntensityPlots

Plotting the intensities of selected beads on a section
numBeads

Gets the number of beads from a beadLevelData object
plotBeadLocations

Plot bead locations
plotMAXY

Scatter plots and MA-plots for all specified arrays
poscontPlot

Plot the positive controls
readTIFF

Read the Illumina tiff images
processSwathData

Prepare iScan data for use with beadarray
readIllumina

Read bead-level Illumina data
readIdatFiles

Read BeadScan gene expression output
readLocsFile

Read ``.locs'' file.
plotTIFF

Produce plots of the Illumina tiff images
readBeadSummaryData

Read BeadStudio gene expression output
summarize

Create a summarized object
transformFunctions

Functions for transforming the data store in a beadLevelData object for easier visualisation or summarisation.
setWeights

Set weights from BASH
show-method

Display object summary
squeezedVarOutlierMethod

Identifier outliers on an array section
weightsOutlierMethod

returns all beads with weight=0.
showArrayMask

Show Array Mask
readSampleSheet

Read a Sample sheet for a BeadArray experiment
sectionNames

Gets the section names from a beadLevelData Object
quickSummary

Create summary values for specified IDs