approx_adjust

Approximates the adjust argument to <code><a href="/link/stats%3A%3Adensity()?package=beadplexr&version=0.5.0" data-mini-rdoc="beadplexr::stats::density()">stats::density()</a></code> needed to find the required number of clusters.

Reproducible and automated analysis of multiplex bead assays such
as CBA (Morgan et al. 2004; <doi: 10.1016/j.clim.2003.11.017>), LEGENDplex
(Yu et al. 2015; <doi: 10.1084/jem.20142318>), and MACSPlex (Miltenyi
Biotec 2014; Application note: Data acquisition and analysis without the
MACSQuant analyzer;
<https://www.miltenyibiotec.com/upload/assets/IM0021608.PDF>). The
package provides functions for streamlined reading of fcs files, and
identification of bead clusters and analyte expression. The package eases
the calculation of standard curves and the subsequent calculation of the
analyte concentration.

Ulrik Stervbo

beadplexr

Analysis of Multiplex Cytometric Bead Assays

approx_adjust function

<dl><dt>.x</dt>
<dd>A numeric vector.</dd>
<dt>.k</dt>
<dd>Numeric giving the number of expected clusters.</dd>
<dt>.lower, .upper</dt>
<dd>The interval for possible value of adjust.</dd>
<dt>.step</dt>
<dd>A numeric giving the increment to adjust. Sometimes low values
are needed to find a proper adjust value.</dd></dl>

Arguments

Approximates the adjust argument to <code><a href='https://rdrr.io/r/stats/density.html'>stats::density()</a></code> needed to find the required number of clusters.

Approximate bandwidth adjustment. — approx_adjust

<dl>

<dt>.x</dt>
<dd>A numeric vector.</dd>


<dt>.k</dt>
<dd>Numeric giving the number of expected clusters.</dd>


<dt>.lower, .upper</dt>
<dd>The interval for possible value of adjust.</dd>


<dt>.step</dt>
<dd>A numeric giving the increment to adjust. Sometimes low values
are needed to find a proper adjust value.</dd>

</dl>

approx_adjust: Approximate bandwidth adjustment.

Description

Usage

Value

Arguments

Details

Examples