fit_standard_curve

Fit a logistic function to the standard concentrations.

Reproducible and automated analysis of multiplex bead assays such
as CBA (Morgan et al. 2004; <doi: 10.1016/j.clim.2003.11.017>), LEGENDplex
(Yu et al. 2015; <doi: 10.1084/jem.20142318>), and MACSPlex (Miltenyi
Biotec 2014; Application note: Data acquisition and analysis without the
MACSQuant analyzer;
<https://www.miltenyibiotec.com/upload/assets/IM0021608.PDF>). The
package provides functions for streamlined reading of fcs files, and
identification of bead clusters and analyte expression. The package eases
the calculation of standard curves and the subsequent calculation of the
analyte concentration.

Ulrik Stervbo

beadplexr

Analysis of Multiplex Cytometric Bead Assays

fit_standard_curve function

<dl><dt>df</dt>
<dd>A tidy data.frame.</dd>
<dt>.parameter</dt>
<dd>A character giving the name of column(s) where populations
are identified.</dd>
<dt>.concentration</dt>
<dd>A character giving the name of the column with the
standard concentration.</dd>
<dt>.fct</dt>
<dd>A character giving the name of the logistic function to use in
the fit, see <code>drc::drm()</code> for details.</dd>
<dt>.data</dt>
<dd>Deprecated. Use <code>df</code>.</dd>
<dt>...</dt>
<dd>Other arguments to <code>drc::drm()</code></dd></dl>

Arguments

Fit a standard curve — fit_standard_curve

<dl>

<dt>df</dt>
<dd>A tidy data.frame.</dd>


<dt>.parameter</dt>
<dd>A character giving the name of column(s) where populations
are identified.</dd>


<dt>.concentration</dt>
<dd>A character giving the name of the column with the
standard concentration.</dd>


<dt>.fct</dt>
<dd>A character giving the name of the logistic function to use in
the fit, see <code>drc::drm()</code> for details.</dd>


<dt>.data</dt>
<dd>Deprecated. Use <code>df</code>.</dd>


<dt>...</dt>
<dd>Other arguments to <code>drc::drm()</code></dd>

</dl>

fit_standard_curve: Fit a standard curve

Description

Usage

Value

Arguments

Examples