The qPCR data set named s1 comprises a six-point, ten-fold dilution series,
replicated in duplicates targeting an amplicon designed with a random
sequence that minimizes secondary structure and off-target primer binding.
Please read the Quantitative PCR data section, qPCR assays subsection of
Boggy et al. (2010) for more details.

datasets

Real-time quantitative polymerase chain reaction (qPCR) data
sets by Boggy et al. (2008) <doi:10.1371/journal.pone.0012355>. This package
provides a dilution series for one PCR target: a random sequence that
minimizes secondary structure and off-target primer binding. The data set
is a six-point, ten-fold dilution series.
For each concentration there are two replicates. Each amplification curve
is 40 cycles long. Original raw data file:
<https://journals.plos.org/plosone/article/file?type=supplementary&id=10.1371/journal.pone.0012355.s004>.

Ramiro Magno

boggy

Real-Time PCR Data Sets by Boggy et al. (2010)

Pattern Institute 

s1 function

A <a href="https://tibble.tidyverse.org/reference/tibble-package.html">tibble</a> with 480 rows and 10 variables:<dl>
<dt><code>plate</code></dt>
<dd>Plate identifier. For this data set it is always missing
(<code>NA</code>).</dd><dt><code>well</code></dt>
<dd>Well identifier.</dd><dt><code>dye</code></dt>
<dd>The type of dye used. In this data set the values are always
<code>"SYTO 13"</code>, meaning 2 mM of the dsDNA dye SYTO-13 (Invitrogen, Carlsbad, CA).</dd><dt><code>target</code></dt>
<dd>Target identifier: the amplicon used, a random sequence
dubbed <code>"S1"</code> by the original authors.</dd><dt><code>sample_type</code></dt>
<dd>Sample type (all curves are standards, i.e. <code>"std"</code>).</dd><dt><code>replicate</code></dt>
<dd>Replicate identifier: either 1 or 2.</dd><dt><code>copies</code></dt>
<dd>Standard copy number.</dd><dt><code>dilution</code></dt>
<dd>Dilution factor. Higher number means greater dilution.</dd><dt><code>cycle</code></dt>
<dd>PCR cycle.</dd><dt><code>fluor</code></dt>
<dd>Raw fluorescence values.</dd>
</dl>

Format

qPCR data set by Boggy et al. (2010) — s1

A <a href='https://tibble.tidyverse.org/reference/tibble-package.html'>tibble</a> with 480 rows and 10 variables:<dl>
<dt><code>plate</code></dt>
<dd>Plate identifier. For this data set it is always missing
(<code>NA</code>).</dd>

<dt><code>well</code></dt>
<dd>Well identifier.</dd>

<dt><code>dye</code></dt>
<dd>The type of dye used. In this data set the values are always
<code>"SYTO 13"</code>, meaning 2 mM of the dsDNA dye SYTO-13 (Invitrogen, Carlsbad, CA).</dd>

<dt><code>target</code></dt>
<dd>Target identifier: the amplicon used, a random sequence
dubbed <code>"S1"</code> by the original authors.</dd>

<dt><code>sample_type</code></dt>
<dd>Sample type (all curves are standards, i.e. <code>"std"</code>).</dd>

<dt><code>replicate</code></dt>
<dd>Replicate identifier: either 1 or 2.</dd>

<dt><code>copies</code></dt>
<dd>Standard copy number.</dd>

<dt><code>dilution</code></dt>
<dd>Dilution factor. Higher number means greater dilution.</dd>

<dt><code>cycle</code></dt>
<dd>PCR cycle.</dd>

<dt><code>fluor</code></dt>
<dd>Raw fluorescence values.</dd>


</dl>

s1: qPCR data set by Boggy et al. (2010)

Description

Arguments

Format

Examples