celltrackR (version 1.1.0)

Neutrophils: Two-Photon Data: Neutrophils in an Infected Lung

Description

Labelled neutrophils were adoptively transfered and intravitally imaged (using two-photon microscopy) inside the lung of the recipient mouse. These cells display a fairly directed kind of motion, as they move towards infection foci.

Usage

data("Neutrophils")

Format

An S3 object of class "tracks"; a list with 10 elements. Each element name identifies a cell track. Each element is a matrix containing the following four columns.

t

the time (in seconds)

x

The X coordinate (in micrometers)

y

The Y coordinate (in micrometers)

z

The Z coordinate (in micrometers)

References

Zinselmeyer BH, Dempster J, Wokosin DL, Cannon JJ, Pless R, Parker I and Miller MJ (2009), Two-photon microscopy and multi-dimensional analysis of cell dynamics. Methods in Enzymology, 461:349--78. doi:10.1016/S0076-6879(09)05416-0

Konjufca V and Miller MJ (2009), Imaging Listeria monocytogenes infection in vivo. Current Topics in Microbiology and Immunology, 334:199--226. doi:10.1007/978-3-540-93864-4_9

Kreisel D, Nava RG, Li W, Zinselmeyer BH, Wang B, Lai J, Pless R, Gelman AE, Krupnick AS, and Miller MJ (2010), In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation. PNAS, 107(42):18073--18078. doi:10.1073/pnas.1008737107

Examples

## load the tracks
data(Neutrophils)
## visualize the tracks (calls function plot.tracks)
plot(Neutrophils)