celltrackR (version 1.2.1)

TCells: Two-Photon Data: T Cells in a Lymph Node

Description

RFP-labelled T cells were injected retro-orbitally in healthy CD11c-YFP mice, and intravitally imaged (using two-photon microscopy) inside a cervical lymph node. These data illustrate the characteristic "random-walk-like" motion pattern of T cells in lymph nodes. For full method details, see references below.

Usage

data("TCells")

Format

## `TCells` An S3 object of class "tracks"; a list with 199 elements. Each element name identifies a cell track. Each element is a matrix containing the following three columns.

t

the time (in seconds)

x

The X coordinate (in micrometers)

y

The Y coordinate (in micrometers)

References

Miller MJ and Wei SH and Parker I and Cahalan MD (2002), Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science, 296(5574):1869--1873. doi:10.1126/science.1070051

Wortel IMN and Liu AY and Dannenberg K and Berry JC and Miller MJ and Textor J (2021), CelltrackR: an R package for fast and flexible analysis of immune cell migration data. ImmunoInformatics, 1-2:100003. doi:10.1016/j.immuno.2021.100003

Examples

  ## load the tracks
  data(TCells)

  ## visualize the tracks (calls function plot.tracks)
  plot(TCells)