C317.melt.hr: Melting Curves (resolution: 0.1 degrees Celsius / step) for the Gene adk Using the Bio-Rad CFX96 thermo cycler

Description

A melting curve for adk was performed.

Usage

data("C317.melt.hr")

Arguments

Format

A data frame with 40 observations on the following 97 variables. The first column ("Temperature") contains the temperature (resolution: 0.1 degrees Celsius / step) and consecutive columns contain the replicates ("A1" to "H12").

Source

Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)

Details

adk was amplified in the Bio-Rad CFX96. After PCR, the temperature-dependent change of fluorescence was simultaneously monitored (EvaGreen, Mao et al. 2007). The primer sequences for adk were taken from this study. adk fw: CTCAGGCTCAGTTCATCATGGA adk rv: AGTTTGCCAGCATCCATAATGTC PCR conditions: C317.amp

References

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33--74, 2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246 Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol. 7, 76.

Examples

Run this code

  data(C317.melt.hr)
  str(C317.melt.hr)

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