C60.melt: Melt Curves MLC-2v and Vimentin for the qPCR Experiment C60.amp Using the Roche Light Cycler 1.5

Description

Melting curves were continuously monitored with the Light Cycler 1.5 (Roche). For details on sample preparation refer to C60.amp.

Usage

data(C60.melt)

Arguments

Format

A data frame with 128 observations on the following 65 variables. Refer to C60.amp for details of the specific samples.

Index: Index of elements
Vim.0.1.T: Temperature
Vim.0.1.F: Fluorescence
Vim.0.2.T: Temperature
Vim.0.2.F: Fluorescence
Vim.1.1.T: Temperature
Vim.1.1.F: Fluorescence
Vim.1.2.T: Temperature
Vim.1.2.F: Fluorescence
Vim.2.1.T: Temperature
Vim.2.1.F: Fluorescence
Vim.2.2.T: Temperature
Vim.2.2.F: Fluorescence
Vim.3.1.T: Temperature
Vim.3.1.F: Fluorescence
Vim.3.2.T: Temperature
Vim.3.2.F: Fluorescence
Vim.4.1.T: Temperature
Vim.4.1.F: Fluorescence
Vim.4.2.T: Temperature
Vim.4.2.F: Fluorescence
Vim.5.1.T: Temperature
Vim.5.1.F: Fluorescence
Vim.5.2.T: Temperature
Vim.5.2.F: Fluorescence
Vim.6.1.T: Temperature
Vim.6.1.F: Fluorescence
Vim.6.2.T: Temperature
Vim.6.2.F: Fluorescence
Vim.7.1.T: Temperature
Vim.7.1.F: Fluorescence
Vim.7.2.T: Temperature
Vim.7.2.F: Fluorescence
MLC2v.1.1.T: Temperature
MLC2v.1.1.F: Fluorescence
MLC2v.1.2.T: Temperature
MLC2v.1.2.F: Fluorescence
MLC2v.2.1.T: Temperature
MLC2v.2.1.F: Fluorescence
MLC2v.2.2.T: Temperature
MLC2v.2.2.F: Fluorescence
MLC2v.3.1.T: Temperature
MLC2v.3.1.F: Fluorescence
MLC2v.3.2.T: Temperature
MLC2v.3.2.F: Fluorescence
MLC2v.4.1.T: Temperature
MLC2v.4.1.F: Fluorescence
MLC2v.4.2.T: Temperature
MLC2v.4.2.F: Fluorescence
MLC2v.5.1.T: Temperature
MLC2v.5.1.F: Fluorescence
MLC2v.5.2.T: Temperature
MLC2v.5.2.F: Fluorescence
MLC2v.6.1.T: Temperature
MLC2v.6.1.F: Fluorescence
MLC2v.6.2.T: Temperature
MLC2v.6.2.F: Fluorescence
MLC2v.7.1.T: Temperature
MLC2v.7.1.F: Fluorescence
MLC2v.7.2.T: Temperature
MLC2v.7.2.F: Fluorescence
MLC2v.0.1.T: Temperature
MLC2v.0.1.F: Fluorescence
MLC2v.0.2.T: Temperature
MLC2v.0.2.F: Fluorescence

Source

Stefan Roediger, Claudia Deutschmann (BTU Cottbus - Senftenberg)

Details

Melting curves were continuously monitored with the Light Cycler 1.5 (Roche).

References

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33--74, 2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246

Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol. 7, 76.

Examples

Run this code

## data(C60.melt)
str(C60.melt)

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