removeNTC

removeNTC-methods

removeNTC,ddCtExpression-method

removeNTC,InputFrame-method


  NTC stands for Non-template controls. This method remove the NTC
  samples from the input object.


methods

The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Jitao David Zhang

ddCt

The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

removeNTC-methods function

<dl> <dt><code>signature(object = "ddCtExpression")</code></dt><dd>
  An object hat has been analyzed with the <code>ddCt</code> method
</dd> <dt><code>signature(object = "InputFrame")</code></dt><dd>
  An input object
</dd> </dl>

Methods

NTC stands for Non-template controls. This method remove the NTC
  samples from the input object.

removeNTC-methods: Remove NTC samples

Description

Arguments

Methods