ddpcr (version 1.11)

classify_droplets: Analysis step: Classify droplets

Description

The main analysis step for ddPCR plates of type pnpp_experiment. Classify each droplet as either rain, ++, or +-. Also calculate the frequency of negative droplets, and attempt to detemine if each well has a statistically significant number of such droplets. See the README for more information about the algorithm used.

Usage

classify_droplets(plate)

Arguments

plate

A ddPCR plate.

Value

A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

See Also

analyze classify_droplets_single mark_clusters has_signif_negative_cluster