The main analysis step for ddPCR plates of type pnpp_experiment
.
Classify each droplet as either rain, ++, or +-. Also calculate the frequency
of negative droplets, and attempt to detemine if each well has a statistically
significant number of such droplets.
See the README for
more information about the algorithm used.
classify_droplets(plate)
A ddPCR plate.
A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze
function) rather than being called
directly.
analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster