ddpcr (version 1.11)

fam_positive_pnpp: Plate type: FAM-positive PNPP

Description

A ddPCR plate of type fam_positive_pnpp, which can also be expressed as (FAM+)/(FAM+HEX+), is a subtype of both pnpp_experiment and wildtype_mutant_pnpp. Use this plate type if your data has three main clusters of droplets: double-negative (empty droplets), FAM+HEX+ (wildtype droplets) and FAM+HEX- (mutant droplets).

Details

Plates with this type have the following analysis steps: INITIALIZE, REMOVE_FAILURES, REMOVE_OUTLIERS, REMOVE_EMPTY, CLASSIFY, RECLASSIFY.

Plates with this type have the following droplet clusters: UNDEFINED, FAILED, OUTLIER, EMPTY (double-negative), RAIN (not empty but not wildtype nor negative), POSITIVE (wildtype), NEGATIVE (mutant).

See the README for more information on plate types.

See Also

plate_types wildtype_mutant_pnpp hex_positive_pnpp analyze remove_failures remove_outliers remove_empty classify_droplets reclassify_droplets

Examples

# NOT RUN {
plate <- new_plate(sample_data_dir(), type = plate_types$fam_positive_pnpp)
type(plate)
plate %>% analyze %>% plot
# }