After classifying droplets into clusters in each well individually, it may be useful to attempt to reclassify droplets in wells where the gates are not very clearly defined. This function uses information taken from wells with a high negative frquency (where the gate is easily defined) to adjust the gates in the other wells. See the README for more information about the algorithm used.
reclassify_droplets(plate)
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze
function) rather than being called
directly.