ddpcr (version 1.11)

remove_failures: Analysis step: Remove failed wells

Description

Check if any wells have failed the ddPCR experiment by checking a series of quality control metrics. If any well is deemed as a failure, all the droplets in that well will be assigned to the FAILED cluster. See the README for more information about the algorithm used to find failed wells.

Usage

remove_failures(plate)

Arguments

plate

A ddPCR plate.

Value

A ddPCR plate with the droplets in failed wells marked as failed. The plate's metadata will have a new variable success which will be FALSE for any failed well and TRUE for all others.

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

See Also

analyze is_well_success