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diffGeneAnalysis (version 1.54.0)

biasAdjust: baisAdjust performs bias adjustment of normalized data

Description

biasAdjust takes a normalized dataset and applies a multiplicative scalar derived from the data to help account for expression biases. These expression biases can come from many sources including dye bias,hybridization efficacy,changes in personnel,etc.After bias adjustment the data is ready for differential gene analysis.

Usage

biasAdjust(normalized, numSlides)

Arguments

normalized
normalized is a matrix of normalized microarray data.The first row consists of headers ans the first column consists of gene names.
numSlides
numSlides is the total number of chips of the microarray dataset.

Value

Returns a matrix of bias adjusted normalized data.

References

Dozmorov I,Centola,M. An associative analysis of gene expression array data. Bioinformatics.2003 Jan22;19(2):204-11

Knowlton N,Dozmorov I, Centola M. Microarray data Analysis Tool box(MDAT): for normalization,adjustment and analysis of gene expression data. Bioonformatics.2004 Dec 12;20(18):3687-90

Examples

Run this code
 
 #biasAdjust(normalized,7)

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