dyebias-package

dyebias


 Many two-colour hybridizations suffer from a dye bias that is both gene-specific and slide-specific. The former depends on the content of the nucleotide used for labeling; the latter depends on the labeling percentage. The slide-dependency was hitherto not recognized, and made addressing the artefact impossible.  Given a reasonable number of dye-swapped pairs of hybridizations, or of same vs. same hybridizations, both the gene- and slide-biases can be estimated and corrected using the GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009), doi:10.1038/msb.2009.21)


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Many two-colour hybridizations suffer from a dye bias that is
both gene-specific and slide-specific. The former depends on the content of
the nucleotide used for labeling; the latter depends on the labeling
percentage. The slide-dependency was hitherto not recognized, and made
addressing the artefact impossible.  Given a reasonable number of
dye-swapped pairs of hybridizations, or of same vs. same hybridizations,
both the gene- and slide-biases can be estimated and corrected using the
GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009),
doi:10.1038/msb.2009.21)

Philip Lijnzaad

The GASSCO method for correcting for slide-dependent gene-specific dye bias

dyebias-package function

Many two-colour hybridizations suffer from a dye bias that is both gene-specific and slide-specific. The former depends on the content of the nucleotide used for labeling; the latter depends on the labeling percentage. The slide-dependency was hitherto not recognized, and made addressing the artefact impossible.  Given a reasonable number of dye-swapped pairs of hybridizations, or of same vs. same hybridizations, both the gene- and slide-biases can be estimated and corrected using the GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009), doi:10.1038/msb.2009.21)

dyebias-package: The GASSCO method for correcting for slide-dependent gene-specific dye bias

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