buildPhylo

Matrix in which each row corrsponds to a copy number segment. Has to contain at least one column for each predicted subpopulation, with columnname labeled SP_xx, where xx is the size of the corresponding SP. Ploidy-matrix can be obtained by calling <code></code>

ploidy

Prefix of file to which phylogeny will be saved.

outF

Neighbor joining algorithm used for phylogeny reconstruction (from library ape). Options: bionjs (default), njs.

treeAlgorithm

Predicts phylogenetic relations between subpopulations from subpopulation specific copy number profiles.

Expanding Ploidy and Allele Frequency on Nested Subpopulations (ExPANdS) characterizes coexisting subpopulations (SPs) in a tumor using copy number and allele frequencies derived from exome- or whole genome sequencing input data. The model amplifies the statistical power to detect coexisting genotypes, by fully exploiting run-specific tradeoffs between depth of coverage and breadth of coverage. ExPANdS predicts the number of clonal expansions, the size of the resulting SPs in the tumor bulk, the mutations specific to each SP and tumor purity. The main function runExPANdS provides the complete functionality needed to predict coexisting SPs from single nucleotide variations (SNVs) and associated copy numbers.  The robustness of the subpopulation predictions by ExPANdS increases with the number of mutations provided. It is recommended that at least 200 mutations are used as an input to obtain stable results. Updates include (1)Phylogeny reconstruction based on SP specific copy number profiles. (2) Additional optional parameter "maxN" provided for function runExPANdS, specifying the maximal number of SNVs to be used for the clustering step. Restriction imposed by "maxN" is now used only for SP detection, not also for mutation assignment to SPs. (3) Relevant for whole genome sequencing (WGS) data: additional optional parameter "region" provided for function runExPANdS, specifying the region within which SNVs are to be used during clustering.

buildPhylo: Relations between subpopulations

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See Also