Easy Michaelis-Menten Enzyme Kinetics
Description
Serves as a platform for published fluorometric enzyme assay
protocols. 'ezmmek' calibrates, calculates, and plots enzyme activities as they
relate to the transformation of synthetic substrates. At present, 'ezmmek'
implements two common protocols found in the literature, and is modular to accommodate
additional protocols. Here, these protocols are referred to as the In-Sample
Calibration (Hoppe, 1983; ) and In-Buffer Calibration (German et al., 2011; ).
protocols. By containing multiple protocols, 'ezmmek' aims to stimulate
discussion about how to best optimize fluorometric enzyme assays. A standardized
approach would make studies more comparable and reproducible.