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flowBin (version 1.8.0)

Combining multitube flow cytometry data by binning

Description

Software to combine flow cytometry data that has been multiplexed into multiple tubes with common markers between them, by establishing common bins across tubes in terms of the common markers, then determining expression within each tube for each bin in terms of the tube-specific markers.

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Version

Version

1.8.0

License

Artistic-2.0

Maintainer

Kieran ONeill

Last Published

February 15th, 2017

Functions in flowBin (1.8.0)

Internal function to map bins by KNN

Calculate the expression of each bin in a BinnedFlowSample in terms of the measurement markers

quantileNormalise

quantileNormalise normalise binning paramaters across all tubes of a flowSample

removeSparseBins

Remove bins from a BinnedFlowSample with few events in them

A class similar to flowSet, but with extra information needed by flowBin

Methods to view flowBin objects

function to run the entire flowBin pipeline

Function to check the quantile normalisation of a FlowSample using flowFP

Bin sample using flowFP binning

Multitube AML sample as example data for flowBin

BinnedFlowSample

A FlowSample, but with binning information for each tube

Count number of events for each tube in each bin

Bin sample using K-means binning