flowQB (version 1.18.0)

LEDflowQBCalculation: QB Calculation for LEDs

Description

This function is used for the LED FCS files to determine, for the channels of interest, the raw statitics for the regression and the generation of the regression's coefficients, Q and B values.

Usage

LEDflowQBCalculation(input, CHANNELTOBEPROCESSED, minF, maxF)

Arguments

input

Folder where the LED FCS files is stored.

CHANNELTOBEPROCESSED

Indices of channels of interest in the LED FCS files.

minF

Minimum MFI to consider for the peaks.

maxF

Maximum MFI to consider for the peaks.

Value

Q and B values with their "Pvalue" & "Std-Error" are in the second list with the coefficients.

Details

The function generates the results as a list, the first element of the list is for raw statistics and the second element of the list is for the coefficients.

References

Faysal El Khettabi et al. 2014, Automated Quadratic Characterization of Flow Cytometer Instrument Sensitivity, to be submitted.

See Also

J. Wood, Fundamental Flow Cytometer Properties Governing Sensitivity and Resolution, Cytometry 33, (1998), p.~ 260 - 6.

E. Chase and R. Hoffman, Resolution of Dimly Fluorescent Particles: a Practical Measure of Fluorescence Sensitivity, Cytometry 33 (1998), p.~ 267-279.

R. Hoffman and J. Wood, Characterization of Flow Cytometer Instrument Sensitivity, Current Protocols in Cytometry, Chapter 1: Unit 1.20 (2007).

A. Gaigalas and L. Wang, Approaches to Quantitation in Flow Cytometry, in Standardization and Quality Assurance in Fluorescence Measurements II Springer Series on Fluorescence (2008), Volume 6, Part D, 371-398.