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flowcatchR (version 1.6.2)

normalizeFrames: Normalize the values of a Frames object

Description

Applies a transformation to the Frames object in a way that the intensities throughout the acquisition are normalized overall in term of pixel values sums. It can be used to compensate for example a global change in the illumination values, e.g. due to changed acquisition conditions in experiments that span long timescales.

Usage

normalizeFrames(frames, normFun = "median")

Arguments

frames
A Frames object to normalize
normFun
The normalization function chosen. Can be one of mean or median

Value

  • A Frames object with normalized pixel values.

Examples

Run this code
data(MesenteriumSubset)
normalizeFrames(MesenteriumSubset,normFun="median")

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