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ggpubr: 'ggplot2' Based Publication Ready Plots

ggplot2 by Hadley Wickham is an excellent and flexible package for elegant data visualization in R. However the default generated plots requires some formatting before we can send them for publication. Furthermore, to customize a ggplot, the syntax is opaque and this raises the level of difficulty for researchers with no advanced R programming skills.

The 'ggpubr' package provides some easy-to-use functions for creating and customizing 'ggplot2'- based publication ready plots.

Find out more at http://www.sthda.com/english/rpkgs/ggpubr.

Installation and loading

  • Install from CRAN as follow:
install.packages("ggpubr")
  • Or, install the latest version from GitHub as follow:
# Install
if(!require(devtools)) install.packages("devtools")
devtools::install_github("kassambara/ggpubr")

Distribution

library(ggpubr)
#> Loading required package: ggplot2
#> Loading required package: magrittr
# Create some data format
# :::::::::::::::::::::::::::::::::::::::::::::::::::
set.seed(1234)
wdata = data.frame(
   sex = factor(rep(c("F", "M"), each=200)),
   weight = c(rnorm(200, 55), rnorm(200, 58)))
head(wdata, 4)
#>   sex   weight
#> 1   F 53.79293
#> 2   F 55.27743
#> 3   F 56.08444
#> 4   F 52.65430

# Density plot with mean lines and marginal rug
# :::::::::::::::::::::::::::::::::::::::::::::::::::
# Change outline and fill colors by groups ("sex")
# Use custom palette
ggdensity(wdata, x = "weight",
   add = "mean", rug = TRUE,
   color = "sex", fill = "sex",
   palette = c("#00AFBB", "#E7B800"))

# Histogram plot with mean lines and marginal rug
# :::::::::::::::::::::::::::::::::::::::::::::::::::
# Change outline and fill colors by groups ("sex")
# Use custom color palette
gghistogram(wdata, x = "weight",
   add = "mean", rug = TRUE,
   color = "sex", fill = "sex",
   palette = c("#00AFBB", "#E7B800"))

Box plots and violin plots

# Load data
data("ToothGrowth")
df <- ToothGrowth
head(df, 4)
#>    len supp dose
#> 1  4.2   VC  0.5
#> 2 11.5   VC  0.5
#> 3  7.3   VC  0.5
#> 4  5.8   VC  0.5

# Box plots with jittered points
# :::::::::::::::::::::::::::::::::::::::::::::::::::
# Change outline colors by groups: dose
# Use custom color palette
# Add jitter points and change the shape by groups
 p <- ggboxplot(df, x = "dose", y = "len",
                color = "dose", palette =c("#00AFBB", "#E7B800", "#FC4E07"),
                add = "jitter", shape = "dose")
 p

 
 # Add p-values comparing groups
 # Specify the comparisons you want
my_comparisons <- list( c("0.5", "1"), c("1", "2"), c("0.5", "2") )
p + stat_compare_means(comparisons = my_comparisons)+ # Add pairwise comparisons p-value
  stat_compare_means(label.y = 50)                   # Add global p-value


 
# Violin plots with box plots inside
# :::::::::::::::::::::::::::::::::::::::::::::::::::
# Change fill color by groups: dose
# add boxplot with white fill color
ggviolin(df, x = "dose", y = "len", fill = "dose",
         palette = c("#00AFBB", "#E7B800", "#FC4E07"),
         add = "boxplot", add.params = list(fill = "white"))+
  stat_compare_means(comparisons = my_comparisons, label = "p.signif")+ # Add significance levels
  stat_compare_means(label.y = 50)                                      # Add global the p-value 

Bar plots

Demo data set

Load and prepare data:

# Load data
data("mtcars")
dfm <- mtcars
# Convert the cyl variable to a factor
dfm$cyl <- as.factor(dfm$cyl)
# Add the name colums
dfm$name <- rownames(dfm)
# Inspect the data
head(dfm[, c("name", "wt", "mpg", "cyl")])
#>                                name    wt  mpg cyl
#> Mazda RX4                 Mazda RX4 2.620 21.0   6
#> Mazda RX4 Wag         Mazda RX4 Wag 2.875 21.0   6
#> Datsun 710               Datsun 710 2.320 22.8   4
#> Hornet 4 Drive       Hornet 4 Drive 3.215 21.4   6
#> Hornet Sportabout Hornet Sportabout 3.440 18.7   8
#> Valiant                     Valiant 3.460 18.1   6

Ordered bar plots

Change the fill color by the grouping variable "cyl". Sorting will be done globally, but not by groups.

ggbarplot(dfm, x = "name", y = "mpg",
          fill = "cyl",               # change fill color by cyl
          color = "white",            # Set bar border colors to white
          palette = "jco",            # jco journal color palett. see ?ggpar
          sort.val = "desc",          # Sort the value in dscending order
          sort.by.groups = FALSE,     # Don't sort inside each group
          x.text.angle = 90           # Rotate vertically x axis texts
          )

Sort bars inside each group. Use the argument sort.by.groups = TRUE.

ggbarplot(dfm, x = "name", y = "mpg",
          fill = "cyl",               # change fill color by cyl
          color = "white",            # Set bar border colors to white
          palette = "jco",            # jco journal color palett. see ?ggpar
          sort.val = "asc",           # Sort the value in dscending order
          sort.by.groups = TRUE,      # Sort inside each group
          x.text.angle = 90           # Rotate vertically x axis texts
          )

Deviation graphs

The deviation graph shows the deviation of quantitatives values to a reference value. In the R code below, we'll plot the mpg z-score from the mtcars dataset.

Calculate the z-score of the mpg data:

# Calculate the z-score of the mpg data
dfm$mpg_z <- (dfm$mpg -mean(dfm$mpg))/sd(dfm$mpg)
dfm$mpg_grp <- factor(ifelse(dfm$mpg_z < 0, "low", "high"), 
                     levels = c("low", "high"))
# Inspect the data
head(dfm[, c("name", "wt", "mpg", "mpg_z", "mpg_grp", "cyl")])
#>                                name    wt  mpg      mpg_z mpg_grp cyl
#> Mazda RX4                 Mazda RX4 2.620 21.0  0.1508848    high   6
#> Mazda RX4 Wag         Mazda RX4 Wag 2.875 21.0  0.1508848    high   6
#> Datsun 710               Datsun 710 2.320 22.8  0.4495434    high   4
#> Hornet 4 Drive       Hornet 4 Drive 3.215 21.4  0.2172534    high   6
#> Hornet Sportabout Hornet Sportabout 3.440 18.7 -0.2307345     low   8
#> Valiant                     Valiant 3.460 18.1 -0.3302874     low   6

Create an ordered barplot, colored according to the level of mpg:

ggbarplot(dfm, x = "name", y = "mpg_z",
          fill = "mpg_grp",           # change fill color by mpg_level
          color = "white",            # Set bar border colors to white
          palette = "jco",            # jco journal color palett. see ?ggpar
          sort.val = "asc",           # Sort the value in ascending order
          sort.by.groups = FALSE,     # Don't sort inside each group
          x.text.angle = 90,          # Rotate vertically x axis texts
          ylab = "MPG z-score",
          xlab = FALSE,
          legend.title = "MPG Group"
          )

Rotate the plot: use rotate = TRUE and sort.val = "desc"

ggbarplot(dfm, x = "name", y = "mpg_z",
          fill = "mpg_grp",           # change fill color by mpg_level
          color = "white",            # Set bar border colors to white
          palette = "jco",            # jco journal color palett. see ?ggpar
          sort.val = "desc",          # Sort the value in descending order
          sort.by.groups = FALSE,     # Don't sort inside each group
          x.text.angle = 90,          # Rotate vertically x axis texts
          ylab = "MPG z-score",
          legend.title = "MPG Group",
          rotate = TRUE,
          ggtheme = theme_minimal()
          )

Dot charts

Lollipop chart

Lollipop chart is an alternative to bar plots, when you have a large set of values to visualize.

Lollipop chart colored by the grouping variable "cyl":

ggdotchart(dfm, x = "name", y = "mpg",
           color = "cyl",                                # Color by groups
           palette = c("#00AFBB", "#E7B800", "#FC4E07"), # Custom color palette
           sorting = "ascending",                        # Sort value in descending order
           add = "segments",                             # Add segments from y = 0 to dots
           ggtheme = theme_pubr()                        # ggplot2 theme
           )

  • Sort in decending order. sorting = "descending".
  • Rotate the plot vertically, using rotate = TRUE.
  • Sort the mpg value inside each group by using group = "cyl".
  • Set dot.size to 6.
  • Add mpg values as label. label = "mpg" or label = round(dfm$mpg).
ggdotchart(dfm, x = "name", y = "mpg",
           color = "cyl",                                # Color by groups
           palette = c("#00AFBB", "#E7B800", "#FC4E07"), # Custom color palette
           sorting = "descending",                       # Sort value in descending order
           add = "segments",                             # Add segments from y = 0 to dots
           rotate = TRUE,                                # Rotate vertically
           group = "cyl",                                # Order by groups
           dot.size = 6,                                 # Large dot size
           label = round(dfm$mpg),                        # Add mpg values as dot labels
           font.label = list(color = "white", size = 9, 
                             vjust = 0.5),               # Adjust label parameters
           ggtheme = theme_pubr()                        # ggplot2 theme
           )

Deviation graph:

  • Use y = "mpg_z"
  • Change segment color and size: add.params = list(color = "lightgray", size = 2)
ggdotchart(dfm, x = "name", y = "mpg_z",
           color = "cyl",                                # Color by groups
           palette = c("#00AFBB", "#E7B800", "#FC4E07"), # Custom color palette
           sorting = "descending",                       # Sort value in descending order
           add = "segments",                             # Add segments from y = 0 to dots
           add.params = list(color = "lightgray", size = 2), # Change segment color and size
           group = "cyl",                                # Order by groups
           dot.size = 6,                                 # Large dot size
           label = round(dfm$mpg_z,1),                        # Add mpg values as dot labels
           font.label = list(color = "white", size = 9, 
                             vjust = 0.5),               # Adjust label parameters
           ggtheme = theme_pubr()                        # ggplot2 theme
           )+
  geom_hline(yintercept = 0, linetype = 2, color = "lightgray")

Cleveland's dot plot

Color y text by groups. Use y.text.col = TRUE.

ggdotchart(dfm, x = "name", y = "mpg",
           color = "cyl",                                # Color by groups
           palette = c("#00AFBB", "#E7B800", "#FC4E07"), # Custom color palette
           sorting = "descending",                       # Sort value in descending order
           rotate = TRUE,                                # Rotate vertically
           dot.size = 2,                                 # Large dot size
           y.text.col = TRUE,                            # Color y text by groups
           ggtheme = theme_pubr()                        # ggplot2 theme
           )+
  theme_cleveland()                                      # Add dashed grids

More

Find out more at http://www.sthda.com/english/rpkgs/ggpubr.

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Version

Install

install.packages('ggpubr')

Monthly Downloads

215,599

Version

0.1.6

License

GPL-2

Last Published

November 14th, 2017

Functions in ggpubr (0.1.6)

compare_means

Comparison of Means
bgcolor

Change ggplot Panel Background Color
axis_scale

Change Axis Scale: log2, log10 and more
as_ggplot

Storing grid.arrange() arrangeGrob() and plots
desc_statby

Descriptive statistics by groups
add_summary

Add Summary Statistics onto a ggplot.
diff_express

Differential gene expression analysis results
annotate_figure

Annotate Arranged Figure
background_image

Add Background Image to ggplot2
border

Set ggplot Panel Border Line
ggballoonplot

Ballon plot
ggbarplot

Bar plot
facet

Facet a ggplot into Multiple Panels
font

Change the Appearance of Titles and Axis Labels
get_palette

Generate Color Palettes
get_legend

Extract Legends from a ggplot object
gene_citation

Gene Citation Index
ggboxplot

Box plot
geom_exec

Execute ggplot2 functions
ggdotchart

Cleveland's Dot Plots
ggline

Line plot
ggadd

Add Summary Statistics or a Geom onto a ggplot
ggmaplot

MA-plot from means and log fold changes
ggarrange

Arrange Multiple ggplots
gghistogram

Histogram plot
ggexport

Export ggplots
ggdotplot

Dot plot
ggecdf

Empirical cumulative density function
ggpie

Pie chart
ggerrorplot

Visualizing Error
ggpubr_args

ggpubr General Arguments Description
ggqqplot

QQ Plots
ggdensity

Density plot
ggpaired

Plot Paired Data
ggscatter

Scatter plot
ggpar

Graphical parameters
ggscatterhist

Scatter Plot with Marginal Histograms
gradient_color

Set Gradient Color
ggstripchart

Stripcharts
ggtexttable

Draw a Textual Table
show_line_types

Line types available in R
ggtext

Text
rremove

Remove a ggplot Component
ggparagraph

Draw a Paragraph of Text
rotate

Rotate a ggplot Horizontally
show_point_shapes

Point shapes available in R
ggviolin

Violin plot
stat_mean

Draw group mean points
stat_chull

Plot convex hull of a set of points
stat_stars

Add Stars to a Scatter Plot
stat_compare_means

Add Mean Comparison P-values to a ggplot
set_palette

Set Color Palette
rotate_axis_text

Rotate Axes Text
text_grob

Create a Text Graphical object
stat_conf_ellipse

Plot confidence ellipses.
theme_pubr

Publication ready theme
stat_cor

Add Correlation Coefficients with P-values to a Scatter Plot
grids

Add Grids to a ggplot
theme_transparent

Create a ggplot with Transparent Background