IgG_inhibition

This data set is for a set of quantitative real-time PCR runs that were
performed in the presence of an optimal amplification reaction mix added with
serial dilutions of IgG (0.0, 0.25, 0.50, 1.0, and 2.0 \(\mu g/ml\)) thus acting as
the inhibitory agent. There are two replicates for each concentration of IgG.
The concentration of the amplicon ND1/ND2 is 41,700,000 copies.
Please read the Methods section of Guescini et al. (2008) for more details.

datasets

Real-time quantitative polymerase chain reaction (qPCR) data
by Guescini et al. (2008) <doi:10.1186/1471-2105-9-326> in tidy format.
This package provides two data sets where the amplification efficiency has
been modulated: either by changing the amplification mix concentration, or
by increasing the concentration of IgG, a PCR inhibitor.
Original raw data files:
<https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-9-326/MediaObjects/12859_2008_2311_MOESM1_ESM.xls> and
<https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-9-326/MediaObjects/12859_2008_2311_MOESM5_ESM.xls>.

Ramiro Magno

guescini

Real-Time PCR Data Sets by Guescini et al. (2008)

Pattern Institute 

IgG_inhibition function

A <a href="https://tibble.tidyverse.org/reference/tibble-package.html">tibble</a> with 400 rows and 10 variables:<dl>
<dt><code>plate</code></dt>
<dd>Plate identifier.</dd><dt><code>well</code></dt>
<dd>Well identifier. Values are always <code>NA</code> (not available). This
variable is kept nevertheless to be coherent with other data sets from other
similar R data packages.</dd><dt><code>dye</code></dt>
<dd>The type of dye used. In this data set the values are always
<code>"SYBR"</code>, meaning SYBR Green I master mix (Roche).</dd><dt><code>target</code></dt>
<dd>Target identifier: the amplicon used, <code>"MT_ND1"</code>.</dd><dt><code>sample_type</code></dt>
<dd>Sample type (all curves are standards, i.e. <code>"std"</code>).</dd><dt><code>replicate</code></dt>
<dd>Replicate identifier: 1 thru 3.</dd><dt><code>IgG_conc</code></dt>
<dd>IgG concentration in \(\mu g/ml\).</dd><dt><code>copies</code></dt>
<dd>Standard copy number.</dd><dt><code>cycle</code></dt>
<dd>PCR cycle.</dd><dt><code>fluor</code></dt>
<dd>Raw fluorescence values.</dd>
</dl>

Format

IgG inhibition — IgG_inhibition

A <a href='https://tibble.tidyverse.org/reference/tibble-package.html'>tibble</a> with 400 rows and 10 variables:<dl>
<dt><code>plate</code></dt>
<dd>Plate identifier.</dd>

<dt><code>well</code></dt>
<dd>Well identifier. Values are always <code>NA</code> (not available). This
variable is kept nevertheless to be coherent with other data sets from other
similar R data packages.</dd>

<dt><code>dye</code></dt>
<dd>The type of dye used. In this data set the values are always
<code>"SYBR"</code>, meaning SYBR Green I master mix (Roche).</dd>

<dt><code>target</code></dt>
<dd>Target identifier: the amplicon used, <code>"MT_ND1"</code>.</dd>

<dt><code>sample_type</code></dt>
<dd>Sample type (all curves are standards, i.e. <code>"std"</code>).</dd>

<dt><code>replicate</code></dt>
<dd>Replicate identifier: 1 thru 3.</dd>

<dt><code>IgG_conc</code></dt>
<dd>IgG concentration in \(\mu g/ml\).</dd>

<dt><code>copies</code></dt>
<dd>Standard copy number.</dd>

<dt><code>cycle</code></dt>
<dd>PCR cycle.</dd>

<dt><code>fluor</code></dt>
<dd>Raw fluorescence values.</dd>


</dl>

IgG_inhibition: IgG inhibition

Description

Arguments

Format

Examples