The function heatmap3 is completely compatible with the original R function heatmap, and provides more new features.
heatmap3(x, Rowv = NULL, Colv = if (symm) "Rowv" else NULL,
distfun = function(x) as.dist(1 - cor(t(x), use = "pa")),
balanceColor = F, ColSideLabs, RowSideLabs, showColDendro = T,
showRowDendro = T, col = colorRampPalette(c("navy", "white",
"firebrick3"))(1024), legendfun, method = "complete", ColAxisColors = 0,
RowAxisColors = 0, hclustfun = hclust, reorderfun = function(d, w)
reorder(d, w), add.expr, symm = FALSE, revC = identical(Colv, "Rowv"),
scale = c("row", "column", "none"), na.rm = TRUE, ColSideFun, ColSideAnn,
ColSideWidth = 0.4, ColSideCut, colorCell, highlightCell,
file = "heatmap3.pdf", topN = NA, filterFun = sd, margins = c(5, 5),
ColSideColors, RowSideColors, cexRow = 0.2 + 1/log10(nrow(x)),
cexCol = 0.2 + 1/log10(ncol(x)), lasRow = 2, lasCol = 2,
labRow = NULL, labCol = NULL, main = NULL, xlab = NULL, ylab = NULL,
keep.dendro = FALSE, verbose = getOption("verbose"), useRaster = if
(ncol(x) * nrow(x) >= 50000) TRUE else FALSE, ...)numeric matrix of the values to be plotted.
determines if and how the row dendrogram should be
computed and reordered. Either a dendrogram or a
vector of values used to reorder the row dendrogram or
NA to suppress any row dendrogram (and reordering) or
by default, NULL, see ‘Details’ below.
determines if and how the column dendrogram should be
reordered. Has the same options as the Rowv argument above and
additionally when x is a square matrix, Colv =
"Rowv" means that columns should be treated identically to the
rows (and so if there is to be no row dendrogram there will not be a
column one either).
function used to compute the distance (dissimilarity)
between both rows and columns. Defaults to dist.
logical indicating if the colors need to be balanced so that the median color will represent the 0 value. The default value is F.
label for ColSideColors
label for RowSideColors
logical indicating if the coloum dendrogram should be plotted (when Colv isn't NA).
logical indicating if the row dendrogram should be plotted (when Rowv isn't NA).
specifying the colors, used in image function.
function used to generate legend in top left of the figure. If not specified, the color bar will be plotted. The users can use any plot functions to generate their own legend. Or a function showLegend is also provided as a example.
the agglomeration method to be used by hclust function. This should be (an unambiguous abbreviation of) one of "ward", "single", "complete", "average", "mcquitty", "median" or "centroid".
integer indicating which coloum of ColSideColors will be used as colors for labels in coloum axis. The default value is 0, which means all coloum labels will be in black color.
integer indicating which coloum of RowSideColors will be used as colors for labels in row axis. The default value is 0, which means all row labels will be in black color.
function used to compute the hierarchical clustering
when Rowv or Colv are not dendrograms. Defaults to
hclust. Should take as argument a result of distfun
and return an object to which as.dendrogram can be applied.
function(d, w) of dendrogram and weights for
reordering the row and column dendrograms. The default uses
reorder.dendrogram.
expression that will be evaluated after the call to
image. Can be used to add components to the plot.
logical indicating if x should be treated
symmetrically; can only be true when x is a square matrix.
logical indicating if the column order should be
reversed for plotting, such that e.g., for the
symmetric case, the symmetry axis is as usual.
character indicating if the values should be centered and
scaled in either the row direction or the column direction, or
none. The default is "row" if symm false, and
"none" otherwise.
logical indicating whether NA's should be removed.
function used to generate annotation and labeling figure in column side. The users can use any plot functions to generate their own figure. And a function showAnn is also provided as a example.
data frame with continuous and factor variables as annotation information. This parameter will be sorted by coloum dendrogram and then passed to ColSideFun.
numeric the height of column side area, which can be used by ColSideFun function.
numeric the value to be used in cutting coloum dendrogram. The dendrogram and annotation will be divided into different parts and labeled respectively.
A data.frame with 3 columns, indicating which cells will be colored by specific colors. The first column is row index, second column is column index, and the third column is color.
A data.frame with 3 or 4 columns, indicating which cells will be highlighted by rectangles with specific colors. The first column is row index, second column is column index, the third column is color for rectangle border, and the optional forth column is width for rectangle border.
pdf file name, only works when topN was used.
vector a list of numbers. topN genes will be used to generate the heatmaps.
function used to filter genes, such as sd, mean, sum. It will be used in a apply function to caculate for each row.
numeric vector of length 2 containing the margins
(see par(mar = *)) for column and row names, respectively.
(optional) character vector of length ncol(x)
containing the color names for a horizontal side bar that may be used to
annotate the columns of x.
(optional) character vector of length nrow(x)
containing the color names for a vertical side bar that may be used to
annotate the rows of x.
positive numbers, used as cex.axis in for the row or column axis labeling. The defaults currently only use number of rows or columns, respectively.
the style of row or column axis labels.
character vectors with row and column labels to use; these default to rownames(x) or colnames(x), respectively.
main, x- and y-axis titles; defaults to none.
logical indicating if the dendrogram(s) should be
kept as part of the result (when Rowv and/or Colv are
not NA).
logical indicating if information should be printed.
logical; if TRUE a bitmap raster is used to plot the image instead of polygons. The grid must be regular in that case, otherwise an error is raised.
additional arguments passed on to image.
The same return value as hclust function.
# NOT RUN {
#gererate data
set.seed(123456789)
rnormData<-matrix(rnorm(1000), 40, 25)
rnormData[1:15, seq(6, 25, 2)] = rnormData[1:15, seq(6, 25, 2)] + 2
rnormData[16:40, seq(7, 25, 2)] = rnormData[16:40, seq(7, 25, 2)] + 4
colnames(rnormData)<-c(paste("Control", 1:5, sep = ""), paste(c("TrtA", "TrtB"),
rep(1:10,each=2), sep = ""))
rownames(rnormData)<-paste("Probe", 1:40, sep = "")
ColSideColors<-cbind(Group1=c(rep("steelblue2",5), rep(c("brown1", "mediumpurple2"),10)),
Group2=sample(c("steelblue2","brown1", "mediumpurple2"),25,replace=TRUE))
colorCell<-data.frame(row=c(1,3,5),col=c(2,4,6),color=c("green4","black","orange2"),
stringsAsFactors=FALSE)
highlightCell<-data.frame(row=c(2,4,6),col=c(1,3,5),color=c("black","green4","orange2"),
lwd=1:3,stringsAsFactors=FALSE)
#A simple example
heatmap3(rnormData,ColSideColors=ColSideColors,showRowDendro=FALSE,colorCell=colorCell,
highlightCell=highlightCell)
#A more detail example
ColSideAnn<-data.frame(Information=rnorm(25),Group=c(rep("Control",5), rep(c("TrtA", "TrtB"),10)))
row.names(ColSideAnn)<-colnames(rnormData)
RowSideColors<-colorRampPalette(c("chartreuse4", "white", "firebrick"))(40)
result<-heatmap3(rnormData,ColSideCut=1.2,ColSideAnn=ColSideAnn,ColSideFun=function(x)
showAnn(x),ColSideWidth=0.8,RowSideColors=RowSideColors,col=colorRampPalette(c("green",
"black", "red"))(1024),RowAxisColors=1,legendfun=function() showLegend(legend=c("Low",
"High"),col=c("chartreuse4","firebrick")),verbose=TRUE)
#annotations distribution in different clusters and the result of statistic tests
result$cutTable
# }