qc.stats: Calculate the number of UMIs and genes per cell and percentage of mitochondrial genes per cell and cell cycle genes.
Description
This function takes data frame and calculates the number of UMIs, genes per cell and percentage of mitochondrial genes per cell and cell cycle genes.
Usage
qc.stats(
x = NULL,
which.data = "raw.data",
mito.genes = NULL,
s.phase.genes = s.phase,
g2m.phase.genes = g2m.phase
)
Arguments
x
A data frame containing gene counts for cells.
which.data
Choose from "raw.data" or "main.data", "imputed.data", default = "raw.data".
mito.genes
A character vector of mitochondrial genes names , default is the genes starting with mt.
s.phase.genes
A character vector of gene names for S phase, default = s.phase.
g2m.phase.genes
A character vector of gene names for G2 and M phase, default = g2m.phase.
Value
The data frame object
Examples
Run this code# NOT RUN {
New.demo.obj <- qc.stats(demo.obj)
head(New.demo.obj@stats)
# }
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