inSilicoMerging (version 1.16.0)

plotRLE: Create RLE plot from (merged) ExpressionSet

Description

Create relative log expression (RLE) plot from ExpressionSet. RLE plots were initially proposed to measure the overall quality of a dataset but can also be used to visualize the presence of unwanted batch effects in the data.

Usage

plotRLE(eset, colLabel, legend=TRUE, file=NULL, ...)

Arguments

eset

ExpressionSet object.

colLabel

colname in pData(eset) to retrieve information for the labeling of samples with a color. All samples with the same value in pData(eset)[,colLabel] will share the same color.

legend

If TRUE a legend will be provided next to the RLE plot.

file

If defined, the resulting plot will be stored as a pdf file instead of shown interactively.

...

Additional parameters for the 'plot' function (e.g. 'main').

Examples

# retrieve two datasets:
library(inSilicoDb);
InSilicoLogin("[e-mail]", "5c4d0b231e5cba4a0bc54783b385cc9a");
eset1 = getDataset("GSE18842", "GPL570", norm="FRMA", features="gene");
eset2 = getDataset("GSE31547", "GPL96",  norm="FRMA", features="gene");
esets = list(eset1,eset2);

# merge them using no additional merging technique and the 'COMBAT' method:
library(inSilicoMerging)
eset_FRMA = merge(esets);
eset_COMBAT = merge(esets, method="COMBAT");

# check available annotations:
colnames(pData(eset_FRMA))
table(pData(eset_FRMA)[,"Disease"]);
table(pData(eset_FRMA)[,"Study"]);

# Visual inspection of the two merged datasets through an MDS plot
plotRLE(eset_FRMA, colLabel="Disease")
plotRLE(eset_COMBAT, colLabel="Disease")