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infercnv (version 0.8)

spike_in_variation_chrs: spike_in_variation_chrs()

Adds a 'SPIKE'-in to the observations set at different thresholds of loss/gain to aid in tracking the effect of infercnv operations and for defining the final scaling.

Usage

spike_in_variation_chrs(infercnv_obj, spike_in_chrs = NULL,
  spike_in_multiplier_vec = c(0.01, 2), max_cells = 100,
  min_genes_per_chr = 100)

Arguments

infercnv_obj

An infercnv object populated with raw count data

spike_in_chrs

: define the chromsomes that will serve as signal for gain/loss default: picks chrosomes in order of size

spike_in_multiplier_vec

: factors that define relative expression for gain/loss and must match ordering of spike_in_chrs above default: c(0.01, 2.0)

max_cells

max number of cells to incorporate in the spike-in

min_genes_per_chr

: default 100