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iq (version 1.10.1)

Protein Quantification in Mass Spectrometry-Based Proteomics

Description

An implementation of the MaxLFQ algorithm by Cox et al. (2014) in a comprehensive pipeline for processing proteomics data in data-independent acquisition mode (Pham et al. 2020 ). It offers additional options for protein quantification using the N most intense fragment ions, using all fragment ions, and a wrapper for the median polish algorithm by Tukey (1977, ISBN:0201076160). In general, the tool can be used to integrate multiple proportional observations into a single quantitative value.

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Install

install.packages('iq')

Monthly Downloads

790

Version

1.10.1

License

BSD_3_clause + file LICENSE

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Maintainer

Thang Pham

Last Published

December 4th, 2024

Functions in iq (1.10.1)

fast_preprocess

Data filtering and normalization
create_protein_list

Creating a list of matrices of fragment ion intensities for all proteins
extract_annotation

Protein annotation extraction
fast_MaxLFQ

The MaxLFQ algorithm
maxLFQ

The MaxLFQ algorithm for protein quantification
plot_protein

Plotting the underlying quantitative data for a protein
median_polish

A wrapper for the R implementation of the median polish algorithm
meanInt

The meanInt algorithm for protein quantification
create_protein_table

Protein quantification for a list of proteins
process_wide_format

Merging rows with identical values in a particular column in a table
spikeins

An example dataset of 12 spike-in proteins
fast_read

Reading data from an input file
preprocess

Data preprocessing for protein quantification
process_long_format

Long format to a wide format table using the MaxLFQ algorithm
topN

The topN algorithm for protein quantification