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iq (version 1.9.12)

Protein Quantification in Mass Spectrometry-Based Proteomics

Description

An implementation of the MaxLFQ algorithm by Cox et al. (2014) in a comprehensive pipeline for processing proteomics data in data-independent acquisition mode (Pham et al. 2020 ). It offers additional options for protein quantification using the N most intense fragment ions, using all fragment ions, and a wrapper for the median polish algorithm by Tukey (1977, ISBN:0201076160). In general, the tool can be used to integrate multiple proportional observations into a single quantitative value.

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install.packages('iq')

Monthly Downloads

1,907

Version

1.9.12

License

BSD_3_clause + file LICENSE

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Maintainer

Thang Pham

Last Published

December 6th, 2023

Functions in iq (1.9.12)

extract_annotation

Protein annotation extraction
median_polish

A wrapper for the R implementation of the median polish algorithm
fast_preprocess

Data filtering and normalization
create_protein_list

Creating a list of matrices of fragment ion intensities for all proteins
maxLFQ

The MaxLFQ algorithm for protein quantification
meanInt

The meanInt algorithm for protein quantification
create_protein_table

Protein quantification for a list of proteins
fast_MaxLFQ

The MaxLFQ algorithm
fast_read

Reading data from an input file
plot_protein

Plotting the underlying quantitative data for a protein
process_wide_format

Merging rows with identical values in a particular column in a table
spikeins

An example dataset of 12 spike-in proteins
preprocess

Data preprocessing for protein quantification
topN

The topN algorithm for protein quantification
process_long_format

Long format to a wide format table using the MaxLFQ algorithm