limma (version 3.28.14)

readHeader: Read Header Information from Microarray Raw Data File

Description

Read the header information from a microarray raw data file, as output from an image analysis software program such as GenePix. These functions are used internally by read.maimages and are not usually called directly by users.

Usage

readGenericHeader(file, columns, sep="\t") readGPRHeader(file) readSMDHeader(file)

Arguments

file
character string giving file name. If it does not contain an absolute path, the file name is relative to the current working directory.
columns
character vector specifying data column headings expected to be in file
sep
the character string separating column names

Value

A list with components corresponds to lines of header information. A key component is NHeaderRecords which gives the number of lines in the file before the intensity data begins. All other components are character vectors.

Details

Raw data files exported by image analysis programs include a number of header lines which contain information about the scanning process. This function extracts that information and locates the line where the intensity data begins. readGPRHeader is for GenePix output and readSMDHeader is for files from the Stanford Microarray Database (SMD). readGenericHeader finds the line in the file on which the data begins by searching for specified column headings.

References

See http://www.cryer.co.uk/file-types/a/atf/genepix_file_formats.htm for GenePix formats.

See http://smd.princeton.edu for the SMD.

See Also

read.maimages

An overview of LIMMA functions to read data is given in 03.ReadingData.