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limma (version 3.28.6)

anova.MAList-method: ANOVA Table - method

Description

Analysis of variance method for objects of class MAList. Produces an ANOVA table useful for quality assessment by decomposing between and within gene sums of squares for a series of replicate arrays. This method produces a single ANOVA Table rather than one for each gene and is not used to identify differentially expressed genes.

Arguments

Usage

anova(object,design=NULL,ndups=2,...)

Details

This function aids in quality assessment of microarray data and in the comparison of normalization methodologies. It applies only to replicated two-color experiments in which all the arrays are hybridized with the same RNA targets, possibly with dye-swaps, so the design matrix should have only one column. The function has not been heavily used and is somewhat experimental.

Value

An object of class anova containing rows for between genes, between arrays, gene x array interaction, and between duplicate with array sums of squares. Variance components are estimated for each source of variation.

See Also

MAList-class, bwss.matrix, anova. An overview of quality assessment and diagnostic functions in LIMMA is given by 09.Diagnostics.