nandb v2.0.7

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Number and Brightness Image Analysis

Calculation of molecular number and brightness from fluorescence microscopy image series. The software was published in a 2016 paper <doi:10.1093/bioinformatics/btx434>. The seminal paper for the technique is Digman et al. 2008 <doi:10.1529/biophysj.107.114645>. A review of the technique was published in 2017 <doi:10.1016/j.ymeth.2017.12.001>.

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nandb

Calculation of molecular number and brightness from fluorescence microscopy image series. The software was published in a 2016 paper. The seminal paper for the technique is Digman et al. 2008. A review of the technique was published in 2017.

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Installation

You can install the release version of nandb from CRAN with:

install.packages("nandb")

You can install the (unstable) development version of nandb from GitHub with:

devtools::install_github("rorynolan/nandb")

I highly recommend using the release version. The dev version is just for the ultra-curious and should be thought of as unreliable.

How to use the package

See the package website at https://rorynolan.github.io/nandb.

Contribution

Contributions to this package are welcome. The preferred method of contribution is through a github pull request. Feel free to contact me by creating an issue. Please note that this project is released with a Contributor Code of Conduct. By participating in this project you agree to abide by its terms.

Functions in nandb

Name Description
cc_brightness_folder Cross-correlated brightness calculations for every image in a folder.
number_timeseries Create a number time-series.
number_folder Number calculations for every image in a folder.
cross_var_pillars Calculate the cross-variance of corresponding pillars of 3d arrays.
cross_var Calculate the cross-variance of two vectors.
cc_number_timeseries_folder Cross-correlated number time-series calculations for every image in a folder.
matrix_raster_plot Make a raster plot of a matrix.
number Calculate number from image series.
cc_number_timeseries Create a cross-correlated number time-series.
nb-ts-img-classes Number and brightness time series image classes.
cc_number Cross-correlated number.
median_filter Smooth and median filters with options for handling NAs.
cc_number_folder Cross-correlated number calculations for every image in a folder.
nandb nandb: Number and brightness in R.
nb-img-classes Number and brightness image classes.
number_timeseries_folder Number time-series calculations for every image in a folder.
cc-nb-ts-img-classes Cross-correlated number and brightness time series image classes.
brightness Calculate brightness from image series.
brightness_folder Brightness calculations for every image in a folder.
cc_brightness_timeseries_folder Cross-correlated brightness time-series calculations for every image in a folder.
cc-nb-img-classes Cross-correlated number and brightness image classes.
cc_brightness_timeseries Create a cross-correlated brightness time-series.
cc_brightness Cross-correlated brightness.
brightness_timeseries Create a brightness time-series.
brightness_timeseries_folder Brightness time-series calculations for every image in a folder.
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Vignettes of nandb

Name
batch-mode.Rmd
brightness-timeseries.Rmd
single-images.Rmd
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