nnNorm (version 2.36.0)

detectSpatialBias: Detecting spatial bias within the print-tips of a two channel array

Description

This function allows to identify in two channel batch of arrays, which are the print-tips where spatial bias is present.

Usage

detectSpatialBias(mbatch, corThreshold=0.6)

Arguments

mbatch

A marrayRaw or marrayNorm batch of two channel arrays.

corThreshold

The correlation treshold to be used.

Value

This function returns a list with two matrices. biasRow and biasCol. The rows of these matrices correspond to the print tips counted metaRow wise, and the columns correspond to arrays. Values in these matrices superior to 33 point to print-tips that have more tha a third of the rows (columns) with important spatial bias.

Details

This function computes two matrices: biasRow and biasCol. The elements of these matrices represent the fraction of rows (columns) for which the correlation coefficient between log-ratios, M, and column index (row index) is higher than a user specified treshold (default corThreshold=0.6). The idea here is to see in which print-tip a important fraction of the rows (columns) are highly correlated with the column (row) index. Since some rows (columns) will show positive correlation while the other negative correlation, we are only interested in a sigle direction of the correlation, i.e. either positive or negative.

References

A robust neural networks approach for spatial and intensity dependent normalization of cDNA microarray data, Adi. L. Tarca , Janice. E. K. Cooke, and John Mackay, Bioinformatics, 21, 2005, 2674 - 2683.

See Also

maNormNN

Examples

# detecting spatial bias in swirl data
data(swirl)
# print-tip, intensity and spatial normalization of the first slide in swirl data set
myres<-detectSpatialBias(swirl)