p2.metadata.from.factor

Generate a list metadata structure that can be passed to a
'pagoda2' web object constructor as additional metadata given a named factor

Analyzing and interactively exploring large-scale single-cell RNA-seq datasets. 'pagoda2' primarily performs normalization and differential gene expression analysis, with an interactive application for exploring single-cell RNA-seq datasets. It performs basic tasks such as cell size normalization, gene variance normalization, and can be used to identify subpopulations and run differential expression within individual samples. 'pagoda2' was written to rapidly process modern large-scale scRNAseq datasets of approximately 1e6 cells. The companion web application allows users to explore which gene expression patterns form the different subpopulations within your data. The package also serves as the primary method for preprocessing data for conos, <https://github.com/kharchenkolab/conos>. This package interacts with data available through the 'p2data' package, which is available in a 'drat' repository. To access this data package, see the instructions at <https://github.com/kharchenkolab/pagoda2>. The size of the 'p2data' package is approximately 6 MB.

Evan Biederstedt

pagoda2

Single Cell Analysis and Differential Expression

Nikolas Barkas

Viktor Petukhov

Peter Kharchenko

Simon Steiger

Rasmus Rydbirk

p2.metadata.from.factor function

<dl><dt>metadata</dt>
<dd>named factor with metadata for individual cells, names must correspond to cells</dd>
<dt>displayname</dt>
<dd>character Name to display for the metadata (default=NULL)</dd>
<dt>s</dt>
<dd>numeric Value for rainbow palette (default=1)</dd>
<dt>v</dt>
<dd>numeric Value for rainbow palette (default=1)</dd>
<dt>start</dt>
<dd>numeric Starting value (default=0)</dd>
<dt>end</dt>
<dd>numeric Ending value (default=NULL)</dd>
<dt>pal</dt>
<dd>optional vector of colours to use, if provided overrides s,v,start and end parameters (default=NULL)</dd></dl>

Arguments

Generate a list metadata structure that can be passed to a
'pagoda2' web object constructor as additional metadata given a named factor — p2.metadata.from.factor

<dl>

<dt>metadata</dt>
<dd>named factor with metadata for individual cells, names must correspond to cells</dd>


<dt>displayname</dt>
<dd>character Name to display for the metadata (default=NULL)</dd>


<dt>s</dt>
<dd>numeric Value for rainbow palette (default=1)</dd>


<dt>v</dt>
<dd>numeric Value for rainbow palette (default=1)</dd>


<dt>start</dt>
<dd>numeric Starting value (default=0)</dd>


<dt>end</dt>
<dd>numeric Ending value (default=NULL)</dd>


<dt>pal</dt>
<dd>optional vector of colours to use, if provided overrides s,v,start and end parameters (default=NULL)</dd>

</dl>

p2.metadata.from.factor: Generate a list metadata structure that can be passed to a 'pagoda2' web object constructor as additional metadata given a named factor

Description

Usage

Value

Arguments