Two tandem mass spec meassured on a linear ion trap coupled to an Orbitrap
mass analyzer (Thermo Scientific, Bremen, Germany). The protein sample originates from 10 mouse brain homogenate, prepared in sterile PBS containing protease
inhibitors (Complete; Riche, Switzerland) by repeated extrusion through
syringe needles of successive smaller size. To remove gross cellular
debris the homogenate was centrifuged for 10 min at 2400 rpm
at 4 degrees centigrade.
The data were acquired withing the Sixth Framework Programm (FP6-37457). The project was called SysProt.