myoglobin

<p>This example data is loosely based on the predicted MALDI-TOF mass list of tryptic peptides of equine myoglobin, created using entry P68082 (MYG_HORSE) in UniProtKB (https://www.uniprot.org/uniprot/P68082) with further modification. Labeled peptides, either singly or doubly dimethylated, present 28 or 56 Da shfit relative to the control.</p>

datasets

In a typical protein labelling procedure, proteins are chemically tagged with a functional group, usually at specific sites, then digested into peptides, which are then analyzed using matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF MS) to generate peptide fingerprint. Relative to the control, peptides that are heavier by the mass of the labelling group are informative for sequence determination. Searching for peptides with such mass shifts, however, can be difficult. This package, designed to tackle this inconvenience, takes as input the mass list of two or multiple MALDI-TOF MS mass lists, and makes pairwise comparisons between the labeled groups vs. control, and restores centroid mass spectra with highlighted peaks of interest for easier visual examination. Particularly, peaks differentiated by the mass of the labelling group are defined as a <e2><80><9c>pair<e2><80><9d>, those with equal masses as a <e2><80><9c>match<e2><80><9d>, and all the other peaks as a <e2><80><9c>mismatch<e2><80><9d>.For more bioanalytical background information, refer to following publications: Jingjing Deng (2015) <doi:10.1007/978-1-4939-2550-6_19>; Elizabeth Chang (2016) <doi:10.7171/jbt.16-2702-002>.

Bo Yuan

protag

Search Tagged Peptides & Draw Highlighted Mass Spectra

myoglobin function

<p>a tibble dataset of 193 rows and 7 variables.</p>

myoglobin: Simulated MALDI-TOF data of equine myoglobin, labeled (with dimethylation) and control

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References