path to the file where the fasta alignment should be saved, if any.
inhouse
logical, if TRUE the in-house MUSCLE software is used. It must be installed on your system and in the search path for executables.
Value
Returns a list of four elements. The first one ($seq) provides the sequences analyzed, the second element ($ids) returns the identifiers, the third element ($aln) provides the alignment in fasta format and the fourth element ($ali) gives the alignment in matrix format.