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pulseTD_0.1.0

Information

Package: pulseTD

Type: Package

Author: XinWang

Maintainer: The package maintainer xindd_2014@163.com

Title: Identification of Transcriptional Dynamics using Pulse Models via 4su-Seq Data and RNA-Seq Data

Version: 0.1.0

Date: 2019-5-5

Description: This package, based on 4sU-seq data and RNA-seq data, analyzes the transcription, processing and degradation rates of genes. pulseTD can not only recognize the transcriptional dynamic rate of the measurement time points, but also obtain continuous changes in transcriptional dynamics. More importantly, it is able to predict the trend of mRNA transcription and expression changes in the future. In terms of performance, pulseTD has better robustness and accuracy than other methods.

License: GPL-2

Encoding: UTF-8

Depends: R (>= 3.4.0)

Imports: AnnotationDbi,SummarizedExperiment,Rsamtools,Biobase,S4Vectors,methods,parallel,GenomicFeatures, ggplot2, grid, GenomicAlignments

RoxygenNote: 6.1.1

Suggests: knitr, rmarkdown, TxDb.Hsapiens.UCSC.hg19.knownGene

VignetteBuilder: knitr

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Install

install.packages('pulseTD')

Monthly Downloads

22

Version

0.1.0

License

GPL-2

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Maintainer

XinWang

Last Published

May 17th, 2019

Functions in pulseTD (0.1.0)

estimateParams

Estimated pulse model parameters!
rpkmSim

A simulated gene expressing Rdata file that contains expression data for total RNA, precursor RNA, and 4sU-labeled RNA.
splitB4Params

esitmate degradation parameters
splitC5Params

esitmate processing parameters
integrateAC

integral function
plotRates,pulseTDmodel-method

plotRate-Method
correctionParams

Correct the parameters of the pulse model
estimateExpression

Estimated Expression
predictExpression,pulseTDmodel-method

predictExpression-method
predictExpression

predict Expression
plotRates

draw Method
preExp

A gene list that stores predicted values of gene expression and a 0.95 confidence interval.
getParams

Get pulseModel params
getRates,pulseTDmodel-method

getRates-method
rpkmres

a list containing the expression values of exons and introns
getRates

Get rate values
splitA6Params

esitmate transcription parameters
integrateA

integral function
pulseModel

pulse function
pulseRates

A pulseTDModel Rdata file containing 100 gene expression data, transcriptional dynamic data already related parameters.
getParams,pulseTDmodel-method

getParams-method
pulseTDmodel-class

pulseTD-model