qpcR (version 1.0-6)

S27: PCR data from 15 different testicular biopsies (Roche Lightcycler)

Description

The data was obtained from cDNA reverse transcribed from the isolated RNA of 15 different testicular biopsies.

Usage

data(S27)

format

A data frame with cycle number and 15 different qPCR runs (F1 - F15).

source

Andrej-Nikolai Spiess & Caroline Feig, Department of Andrology, University Hospital Hamburg, Germany.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Examples

data(S27)
### on single data
m1 <- multdrc(F1 ~ Cycles, data = S27, fct = l5())
pcrplot(m1)
### defining 15 replicates
repData <- repform(S27[,1:16], c(0, rep(1, 15)))
m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2, type = "all")