qpcR (version 1.0-6)

pcrpred: Value prediction from a fitted sigmoidal model

Description

After fitting the appropriate sigmoidal model, either the raw fluorescence values can be predicted from the cycle number or vice versa.

Usage

pcrpred(object, newdata, which = c("y", "x"), ...)

Arguments

object

a 'drc' object.

newdata

a vector containing the values to estimate from.

which

either "y" (default) for prediction of the raw fluorescence or "x" for prediction of the cycle number.

...

other parameters to be passed to the predict.drc function.

Value

• A vector containing the estimated values.

Details

Uses the predict.drc function from the 'drc' library for estimation, if the raw fluorescence values are to be estimated, and the inversion function of either the llogistic or bolztmann models if the cycle number is to be estimated.

Examples

m <- multdrc(F1.1 ~ Cycles, data = reps, fct = l5())
### which raw fluorescence value at cycle number = 17?
pcrpred(m, 17)
### cycle numbers 20:25?
pcrpred(m, 20:25)
### which cycle at F = 4
pcrpred(m, 4, "x")