qpcR (version 1.0-6)

reps: qPCR dilution experiment with replicates (Roche Lightcycler)

Description

A dilution experiment with seven 10-fold dilutions of the cDNA, and four replicates for each dilution.

Usage

data(reps)

format

A data frame with the PCR cycles and 28 qPCR runs with four replicates of seven 10-fold dilutions. The replicates are defined by F1.1 - F1.4 (first dilution), F2.1 - F2.4 (second diltution) etc.

source

Andrej-Nikolai Spiess & Nadine Mueller, Institute for Hormone and Fertlity Research, Hamburg, Germany.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Examples

data(reps)
### on single data
m1 <- multdrc(F1.1 ~ Cycles, data = reps, fct = l3())
pcrplot(m1)
### on replicated data
repData <- repform(reps[,1:5], c(0,1,1,1,1))
m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2)