qpcR (version 1.0-6)

reps2: Another qPCR dilution experiment with replicates (Roche Lightcycler)

Description

A dilution experiment of two different cDNAs with five 4-fold dilutions of the cDNA, and three replicates for each dilution.

Usage

data(reps2)

format

A data frame with the PCR cycles and 30 qPCR runs with three replicates of five 4-fold dilutions. The replicates are defined by FX.Y.Z (X = cDNA number, Y = dilution number, Z = replicate number).

source

Heike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Examples

data(reps2)### on single data
m1 <- multdrc(F1.1.1 ~ Cycles, data = reps2, fct = l3())
pcrplot(m1)
### on replicated data
repData <- repform(reps2[,1:4], c(0,1,1,1))
m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2)