qpcR (version 1.0-6)

reps3: A qPCR dilution experiment with replicates (Stratagene MXPro3000P)

Description

A high quality dilution experiment with six 4-fold dilutions of the cDNA, and three replicates for each dilution.

Usage

data(reps3)

format

A data frame with the PCR cycles and 21 qPCR runs with three replicates of six 4-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).

source

Heike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a MXPro3000P instrument (Stratagene). Data was ROX-normalized, but without smoothing.

Examples

data(reps3)### on single data
m1 <- multdrc(F1.1 ~ Cycles, data = reps3, fct = l4())
pcrplot(m1)
### on replicated data
repData <- repform(reps3[,1:4], c(0,1,1,1))
m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2, type = "all")